miR-221, miR-650 and miR-4534 as diagnostic markers in prostate cancer and their relationship with lymphatic invasion

Abstract Objectives This study aimed to examine the role of miR-221, miR-650, and miR-4534 expressions in development, pathogenesis, early diagnosis prostate cancer. Methods The included 83 participants: 37 patients with PCa, 31 BPH, 15 healthy subjects. MiRNA plasma samples was evaluated by quantitative RT-PCR. Results Plasma miR-221 levels were significantly upregulated PCa BPH groups compared control group. A significant difference determined between presence lymph node metastasis miRNAs. In ROC analysis miRNAs, it that AUC for 0.737 a sensitivity 57% specificity 100%, miR-650 0.706 62% 93% 0.800 73% 93%. Conclusions Overexpression may distinguish from controls, but seems be insufficient differentiating when used alone or combination. However, these oncogenic miRNAs have determining development progression disease suppressing tumor suppressor genes they target.